The highly experienced team at the Nucleic Acid PCR Core Facility (NAPCore) provides cutting-edge knowledge coupled with proven “gold standard” technologies that allow investigators to perform sophisticated experiments while saving time and facilitating genomics research and discovery. NAPCore optimizes a suite of state-of-the-art equipment in 2,000 square feet of space, operating as a centralized research resource to meet investigators’ molecular biology research needs — for both small and large projects — with confidence.
The NAPCore staff executes a seamless workflow to streamline basic and translational research, from assistance with experimental design, to preparing samples, to next-generation sequencing, to real-time PCR and data analysis. They are committed to working closely with investigators in multiple fields of science at Children’s Hospital of Philadelphia, the University of Pennsylvania, and external users to advance their research with the following nucleic acids-related platforms and services that generate high quality data:
- Sanger DNA sequencing
- Fluorescent fragment analysis (microsatellite genotyping and human identity)
- NGS sequencing and automated NGS library prep
- Oligonucleotide synthesis (through a partnership with IDT)
- Real-Time PCR services (instrumentation, assay design, and troubleshooting)
- DNA and RNA analysis (quantitation and quality analysis).
As these innovative technologies evolve at a rapid pace, NAPCore staff are continually finding new ways to help researchers yield novel insights into genetic variation and function that will propel pediatric research in new directions.
For general inquiries, scheduling, and for additional information on services please email napcore [at] email.chop.edu; realtime [at] email.chop.edu; or contact the staff representatives listed below.
Eric Rappaport, Scientific Director
3501 Civic Center Boulevard
Colket Translational Research Building, Room A-450
Philadelphia, PA 19104
rappaport [at] email.chop.edu